PHE_Scientific_ Analysis of iPSCs by G-banding

Public Health England (PHE) has awarded a contract to Cell Guidance Systems to assess the chromosomal stability of human induced pluripotent stem cells (iPSCs). The service subject to this contract are: - Be able to receive, handle and process live cells that are Advisory Committee on Dangerous Pathogens (ACDP) Hazard Group 2. - Have experience handling and fixing human induced pluripotent stem cells (iPSCs) e.g. identifying colony cultures that are in the log phase of growth. - Use Colcemid for arresting cells in metaphase so that chromosomes can be separated for cytogenetic studies. - Fix the cells using potassium chloride (KCl) solution and 3:1 methanol: acetic acid. - Assess the karyology of the fixed cells by G-banding - at least 20 G-banded metaphase spreads needed for each sample. - Provide a summary of the representative karyotype, along with additional information if any karyotypic abnormalities are found.